Phospho antibody array
WebAntibody arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. WebApoptosis Phospho Antibody Array features 247 highly specific antibodies related to apoptosis and cell death. The array is designed for protein phosphorylation profiling and …
Phospho antibody array
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WebSep 20, 2024 · The phospho-protein arrays used in our experiments are based on analysis of tissue samples on nitrocellulose membranes, where specific antibodies against selected … WebNov 14, 2024 · The Phospho Explorer Antibody Array is a glass-based antibody array for broad-scope protein phosphorylation profiling and screening in cell and tissue samples …
WebNov 15, 2024 · This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-H2AX and total H2AX. An anti-pan H2AX antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and H2AX present in a sample is bound to the wells by the immobilized antibody. WebOct 16, 2024 · The Proteome Profiler Mouse Phospho-RTK Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1).
Webwith those identified in the phospho-antibody array analysis, we identified 38 protein kinases that are regulated by SII stimulation (Table S3). Included in this group of 38 are kinases in the MAPK Fig. 1. Human phospho-antibody array analysis reveals that phosphoryla-tion events induced by SII are primarily β-arrestin–dependent. (A) Normal- WebAntibody Array Kit for studying IGF1R beta in the research area. ... between lysate protein concentration from untreated and IGF-I treated MCF-7 cells and the relative fluorescence of phospho-IGF-IR (panTyr), phospho-Akt (Ser473) and phospho-p44/42 (Thr202/Tyr204) is shown. MCF-7 cells were starved for 24 hours, then treated with 100 ng/ml IGF ...
WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
WebThis sequence is conserved in human (Ser633) and rat (Ser632) eNOS. WB (1:500). Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. raid shadow legends tier list 2022 excelWebThe antibody array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a … raid shadow legends toragi the frog clan bossWebHuman Phospho-Kinase Antibody Array (Membrane, 43 Targets) (Y0225) (AbAr-0225-YC) Antibody array is a specific form of protein microarray. In this technology, capture … raid shadow legends time limitWebHow to analyze data from protein arrays? I performed the Cell Cycle Control Phospho Antibody Array ( http://www.fullmoonbio.com/product/cell-cycle-control-phospho-antibody-array/) with 7... raid shadow legends tipps deutschraid shadow legends tier list dragonWebCreative Biolabs has developed antibody array technology platform and various antibody array products have been provided. If you have special needs, Creative Biolabs also provides antibody array customization services for your exact applications. Here are what we have and can be provided: Antibody Array Products Membrane Antibody Arrays raid shadow legends tome farmingWebHuman Phosphorylation Pathway Profiling Array C55 Rating: 1 Review 2 Citations Detects 55 phosphorylated human proteins in 5 signaling pathways: MAPK, AKT, JAK/STAT, NFκB, and TGFβ. Suitable for all liquid sample types but intended for use with cell and tissue lysates. MANUAL / SDS $901.00 Size In stock SKU AAH-PPP-1-2 + Add to Cart raid shadow legends torturehelm build