Trizon reagent cwbio
WebDec 15, 2024 · Total RNA from zebrafish brain tissues was extracted with TRIzon Reagent (CWBIO, China). The quality and concentration of extracted RNA were determined by 1.5% … WebMar 1, 2024 · Total RNA was extracted from CytPM-COS and mock treatment N. tobacum K326 using TRIzon Reagent (CWBIO, Beijing, China). Thereafter, cDNA was synthesized …
Trizon reagent cwbio
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WebNov 27, 2024 · For each sample, approximately 2,000 synchronized young adult worms were collected and resuspended in TRIzon Reagent(CW0580, CWBIO). Total RNA was isolated by chloroform extraction, followed by isopropanol precipitation. RNA libraries were prepared for sequencing using standard Illumina protocols. PolyA RNA was selected. Library strategy: … WebNov 26, 2024 · Total RNA was extracted from the samples using TRIzon reagent RNA kit (CWbio). RNA (1 μg) was reverse transcribed with TransScriptR one-step gDNA Removal and cDNA Synthesis SuperMix (Transgen Biotech Co., Ltd.). The …
WebApr 14, 2024 · Every 50 μL PCR assay reaction mixture consisted of 25 μL of 2× PCR premix Taq polymerase (CWBio, Beijing, China), 1 μL of the DNA template, 0.5 μL of each primer, and 21 μL of water. PCR amplification consisted of an initial denaturation step at 94°C for 2 min, followed by 30 cycles of denaturation at 94°C for 1 min, annealing at 66°C ... WebMar 1, 2024 · After cell transfection, total RNA was extracted using Trizon reagent (CW0580, CWBIO, China) and reversely transcribed to cDNA using HiFiScript cDNA synthesis kit (CW2141, CWBIO, China) according to the manufacturer’s instructions.
WebJan 19, 2024 · Many nitrate transporters, transcription factors, and protein kinases participate in the regulation of nitrate signaling. Here, we identified a gene encoding the chrysanthemum calcineurin B-like... WebTRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample …
Web1a.植物组织：取新鲜植物组织在液氮中充分研磨或将植物组织剪碎后直接在TRIzon 中迅速研磨，每30-50 mg组织加入1 ml TRIzon，混匀。 注意：样品体积一般不要超过TRIzon体积 …
WebAkbar Dastjerdi, ... Nicholas Johnson, in Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, 2014. 19.2.4 Nucleic Acid Extraction and DNase Digestion. … sports analysts nfl picksWebDec 8, 2024 · Status: Public on Nov 23, 2024: Title: mRNA-seq of wild-type(N2), hlh-11 knock-out(KO) and hlh-11 over-expression(OE) worms: Organism: Caenorhabditis elegans shelly loweWebSep 3, 2024 · To clarify the crucial differentially expressed genes (DEGs) induced by NNM in the TMV-infected plant cells, total RNA was extracted from NNM and mock treatment BY-2 protoplasts at 20 hpi with a TRIzon Reagent (CWBIO, Beijing, China) according to the manufacturer’s protocol. sports analytics datasetsWebReagent are compatible with phenol, guanidine isothiocyanate, and chloroform. Lyse samples and separate phases 1. Lyse and homogenize samples in TRIzol™ Reagent … sports analytics career pathWebFor each sample, approximately 2,000 synchronized young adult worms were collected and resuspended in TRIzon Reagent(CW0580, CWBIO). Total RNA was isolated by chloroform … shelly lowe facebookWebDec 1, 2024 · 中国病理生理杂志 Chinese Journal of Pathophysiology 2024，38（11）：1921-1928 杂志网址：http: / / www. cjpp. net KLF15基因敲除加重急性心肌梗死 小鼠心肌损伤* 高诗娟， 黄 山， 方光明， 张燕红， 杜 杰 （首都医科大学附属北京安贞医院，北京市心肺血管疾病研究所，北京 100029） [摘 要] 目的：探讨Kruppel样因子 ... shelly lowe chair nehWeb品牌：CWBIO ￥998. 咨询 RNApure Tissue&Cell Kit. 品牌：CWBIO ￥798. 咨询 Universal Genomic DNA Kit. 品牌：CWBIO ￥1298. 咨询 Universal Genomic DNA Kit. 品牌：CWBIO ￥398 ... TRIzon Reagent. 品牌：CWBIO ￥398. sports analytics companies in usa